Staining and biochemical characterization of cellulase producing bacteria. For a rapid screening of cellulase producing bacteria, after the incubation of the agar medium are containing 0. Microbial cellulases production, applications and challenges rajeev k sukumaran, reeta rani singhania and ashok pandey biotechnology division, regional research laboratory, industrial estate p o, trivandrum 695 019 microbial cellulases find applications in various industries and constitute a major group of the industrial enzymes. Fungal cellulase is an abundant resources,is a potential raw material for the microbial production of food, fuel etc. It describes the current knowledge of cellulase production by submerged fermentation and solid state fermentation, properties of cellulase and cloning and expression of cellulase gene. Amylase and cellulase producing fungi can be isolated from various environments such as soil saxena et al. Jul 29, 2009 screening and isolation of cellulase producing bacteria. Sasidharan sreedevi, sreedharan sajith, sailas benjamin enzyme technology laboratory, biotechnology division, department of botany, university of calicut, malappuram, india. Isolation and screening of cellulolytic bacteria from soil. Sasidharan sreedevi, sreedharan sajith, sailas benjamin. Trichoderma virens also known as gliocladium virens, it is a plant protector, enhances biomass production, contrerascornejo et al. Trichoderma species are considered as most suitable candidate for cellulase production and utilization in industry as compared to aspergillus and humicola. Many cellulase producing fungi including trichoderma, penicillium, botrytis neurospora7 23 genra aspergilli 8 24 aspergillus niger and aspergillus.
Among bacterial species, both aerobic and anaerobic organisms are documented for a fairly good amount in all. Phylogenetic diversity and characterization of novel and. It performs hydrolysis of cellulose during drying of coffee beans. Some microbiology project topics and academic research papers. Cellulose is the most abundant organic polymer which may be hydrolyzed by enzyme cellulase into smaller sugar components including glucose subunits which can be used for production of ethanol, organic acids and other chemicals. An overview on fungal cellulases with an industrial perspective. Screening and identification of cellulase producing bacteria.
The microorganism is a mutant strain of an ascomycete fungus capable of synthesizing cellulases. Production of cellulase enzyme from isolated fungus and. Pdf microbial cellulasesproduction, applications and. Fungi are the main cellulase producing microorganisms, though a few bacteria and actinomycetes have also been recently reported to yield cellulase activity table 31. Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production. Isolation and characterization of efficient cellulolytic fungi african. Jain central pulp and paper research institute, saharanpur, u. To screen the best suitable of cellulaseproducing fungi for the industrial uses, three parameters will be considered. In the present investigation, we aim to isolate the two novel cellulase producing fungi aspergillus niger and trichoderma sp. To screen the best suitable of cellulase producing fungi for the industrial uses, three parameters will be considered. The highest occurrence of fungi was recorded from soil samples 52%, followed by saw dust 29%, and least was in decaying wood 19%. Most commonly studied cellulolytic fungi includetrichoderma, humicola, penicillium and aspergillus rana and kaur, 2012. Isolation of amylase and cellulase producing fungi from. For understanding the mechanism of cellulose degradation by cellulase, it is.
The culture conditions like ph, temperature, carbon sources, and nitrogen sources were optimized. Optimum ph for cellulase production by these bacterial isolates was 7. Over the years, culturable, cellulase producing bacteria have been isolated from a wide variety of sources such as composting heaps, decaying plant material from forestry or agricultural waste, the feces of ruminants such as cows, soil and organic matter, and extreme environments like hotsprings, to name a few 24. Fungi are the main cellulase producing microorganisms. Cellulase production from species of fungi and bacteria. The selection of improved producers among the huge number of variants in mutant libraries is a key issue in filamentous fungi of industrial biotechnology.
Pdf cellulase production from species of fungi and. For cellulase production fungi have good capability as compared to bacteria. Maximal cellulase production was obtained after 48 h of incubation at 45 c in medium containing 1. Cellulases are inducible enzymes synthesized by a large diversity of microorganisms. Fungi, cellulase, production, optimization, rsm, application, biofuel. Isolation, screening and identification of cellulase. Many cellulase producing fungi including trichoderma, penicillium, botrytis neurospora7 23 genra aspergilli 8. Cellulase enzymes are isolated from fungi present in higher termite soil.
The prospects of cellulaseproducing bacteria for the. Amylase and cellulaseproducing fungi can be isolated from various environments such as soil saxena et al. Cellulolytic fungi an overview sciencedirect topics. For complete hydrolysis of cellulose to glucose, cellulase systems must contain endo 1, 4 glucan 1, 4 d glucanohydrolase ec. Fungi are the main cellulaseproducing microorganisms, though a few bacteria and actinomycetes have also been recently reported to yield cellulase activity table 31. Screening isolation and characterization of cellulase. Cellulase is a multienzyme,system having 3 types of. Screening of fungi isolated from environmental samples for. The main purpose of this study is to reduce the production cost of cellulase by optimizing the production medium and using an alternative carbon source such as municipal solid waste residue. Cellulase production from species of fungi and bacteria from. Production, optimization and characterization of fungal. Bacteria, having high growth rates as compared to fungi, have better potential for commercial cellulase production.
Other than aspersillus sp, trichoderma is also the best cellulase producing. Isolation and characterization of cellulase producing bacteria from soil basavaraj i. Pdf in energy deficient world, cellulases play a major role for the production of alternative energy resources utilizing lignocellulosic waste. Cellulase producing bacteria from the woodyards on kallai river bank.
Research article cellulase production by aspergillus niger. The present study was aimed to isolate, screen and identify the potential cellulase and xylanase producing fungi from the soil samples collected from different areas of haryana. Some of these produce significant quantities of extracellular enzymes capable of completely hydrolyzing crystalline cellulose in vitro. They are produced by fungi, bacteria, protozoans, plants, and animals. Fungi from all six genera observed were isolated from the soil samples. Among bacteria, bacillus species produce a number of extracellular enzyme including amylases, proteinases, and polysaccharide hydrolases mawadza et al. Deciphering cow dung for cellulase producing bacteria.
Thermophilic fungi as new sources for production of cellulases and. Screening and identification of cellulase producing. Pdf microbiology academic papers and microbiology project. Cellulase production by utilizing agricultural wastes. Cellulase production from bacterial strains were also studied though less in number than fungal cellulase due to lower penetration ability and unavailability to use a wide range of substrates for cellulase production wei et al.
The soil samples were obtained 10gm from hardoi district, uttar pradesh, india. Cellulase and xylanase production sakshi bairagi department of environmental sciences, m. Optimization for the production of cellulase enzyme from. Optimization of cellulase production from bacteria isolated.
Hunt for cellulase producing fungi from soil samples article pdf available in journal of pure and applied microbiology march 2015 with 1,167 reads how we measure reads. Efficient inducers of xylanase production include xylan, xylan hydrolysis intermediates xylobiose, dxylose, lactose, lsorbose, sophorose, and even cellulose xu et al. The synthesis of cellulases by the mutant is nonrepressed by glycerol, repressed by glucose but not subject to postrepression lag, and inducible to high levels by lactose. Optimization of cellulase production from bacteria. This study was conducted to isolate, screening and purification of cellulase from bacteria present in sugar industry waste molasses and characterization by morphological and biochemical analysis.
Cellulase production from fungi is advan tageous as the enzyme production rate is higher compared to other microorganisms. Cellulase enzyme has been used for the bioconversion of lignocellulosic to these useful products. The objective of the present study was to collect the soil sample for isolation and identification of fungi using appropriate method, to screen the cellulase producing. Clear zones surrounded the colonies with zone diameter measuring 12 to 45 mm respectively, and their cellulase. Pdf cellulase production from species of fungi and bacteria from. A novel methodology of isolation and primary screening of cellulolytic fungi was adopted by exposing carboxy methyl.
Mojsov, in new and future developments in microbial biotechnology and bioengineering, 2016. Cellulaseproducing fungi were isolated from different samples like soil, compost, decayed lignocellulosic waste etc using different isolation strategies. Fungi are the main cellulase producing microorganism in which aspergillus sp. Optimization of the fermentation medium for maximum cellulase production was carried out. Optimum level of cellulase was produced by pseudomonas and bacillus isolates at 37c, and that by serratia isolate was at 35c. Isolation and screening of cellulase producing bacteria from cow dung. Production of cellulase was analyzed by dinitrosalicylic acid dns and carboxymethyl cellulase assay methods.
A growing body of evidence shows filamentous fungi employing mechanisms to control the production of cellulolytic enzymes. Pdf cellulase production from species of fungi and bacteria. Microbial cellulases production, applications and challenges article pdf available in journal of scientific and industrial research 6411. No detectable cellulase activity was noted during whole cultivation. Screening isolation and characterization of cellulase producing. Analysis of cellulase and polyphenol oxidase production by. Optimum temperature and ph for the activity of cellulase from these isolates were 40c and 7. Production of cellulase enzyme from isolated fungus and its application as efficient refining aid for production of security paper chetna gupta, priyanka jain, dhermander kumar, a. Cellulase is used for commercial food processing in coffee. Pdf hunt for cellulase producing fungi from soil samples. Seven dominant isolates of cellulase producing fungi were isolated from pulverized rotten wood samples obtained from nnamdi azikiwe university awka botanical garden in anambra state nigeria. Depending on the diameter of clear zone around the colony, three fungal isolates were identified as cellulase producing fungi and its initial identification was done by fungal staining and colony morphology7,8. Klepzig3 1department of biotechnology and 2department of food engineering, middle east technical university, 06531 ankara, turkey, tel. The cellulase activity was assayed by carboxymethyl cellulase cmcase endoglucanase assay.
Study revealed the best condition for cellulase production for fungi was found to be ph 6 with temperature 30c2 and ammonium chloride as nitrogen source. The solid cellulosic waste can be treated efficiently. Ultrahighthroughput picoliterdroplet microfluidics. Here, we developed a dropletbased microfluidic highthroughput screening platform for selection of high cellulase producers from filamentous fungus trichoderma reesei. Maximum growth of the organism was found at 48 h under submerged condition in xylan containing enriched medium, whereas highest enzyme production 4. Research article enhanced production of cellulase on. Cellulase production from species of fungi and bacteria from agricultural wastes and its utilization in industry. Production of cellulase enzyme from isolated fungus and its. Solid state fermentation ssf for the production of.
Microbial cellulases production, applications and challenges. Isolation and characterization of cellulase producing. Screening, purification and characterization of cellulase. A process and a microorganism for synthesizing cellulase enzymes are described. Produces mycotoxins and secondary metabolites that can contaminate animal feed and human foods leslie, 2003. Analysis of cellulase and polyphenol oxidase production by southern pine beetle associated fungi abduvali valiev1, zumrut b.
Screening of cellulase producing fungi was performed on cmc agar plates flooded with congo red and washed with nacl. This is an efficient example of genetic modification and its application for achieving a cellulase hyperproducer in fungi, especially penicillium yao et al. Fungi are the main cellulase producing microorganisms, though a few bacteria and actinomycetes have also been reported to yield cellulase activity. Cellulase is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related polysaccharides. Cellulaseproducing bacteria were isolated from soil and identified as pseudomonas fluorescens, bacillus subtilis, e. Cellulases and xylanases are among the fungal enzymes produced by ssf with potential for industrial applica tions.
Cellulase production from fungi is advantageous as the enzyme production rate is higher compared to other microorganisms. Efficient cellulase producing fungi isolates were finally selected based on the zone of the clearing around the fungi on carboxyl methyl cellulase agar cmc agar plates bakare et al. Out of 21 fungal isolates, only 4 isolate produced zones of hydrolysis in cmc agar plates within 3 days and results were represented fig. A xylanase producing fungi has been isolated from soil and identified as trichoderma reesei saf3. The screening system used a fluorogenic assay to measure amount of. The name is also used for any naturally occurring mixture or complex of various such enzymes, that act serially or synergistically to decompose cellulosic material. Cellulase producing bacteria from the woodyards on kallai. Isolation and screening of cellulolytic fungi semantic scholar. Kaliwal department of studies in biotechnology and microbiology, karnatak university, dharwad 580 003, india corresponding author abstract introduction enzymes are delicate protein molecules necessary for life. Solely, fungi naturally manufacture the require titers of cellulases needed for the entire saccharification 30 50 mg enzymeg of crystalline cellulose 6 22. Microorganisms of the genera trichoderma and aspergillus are thought to be cellulase producers, and crude enzymes produced by these microorganisms are commercially available for.
Glucosidase and it work together with endoglucanase and to break down cellulose in the plant cell walls. Isolation, screening and selection of fungal strains for. Screening and identification of cellulase producing bacteria isolated from oil palm meal saowapar khianngam1, yupa pootaengon1, taweesak techakriengkrai2, somboon tanasupawat3 1faculty of animal sciences and agricultural technology, silpakorn university, phetchaburi it campus, phetchaburi 76120, thailand. Presently, work is aimed at screening and isolating cellulolytic fungi from the soil samples collected from 2 different areas of himachal. Cellulase producing bacteria were isolated from soil and identified as pseudomonas fluorescens, bacillus subtilis, e. The samples were grown in czapex dox agar medium incorporated with carboxymethylcellulose.
Although the application of bacteria in producing cellulase is less investigated, cellulases produced by bacteria are often more e. The production of biobased products and bioenergy from less costly renewable lignocellulosic materials would bring benefits to the local economy, environment, and national energy security zhang, 2008. Introduction cellulose are produced mainly in plants. The greater the diameter of clear zone, the larger amount of carboxymethylcellulose cmc can be degraded. Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose cmc agar plate were identified as cellulase producing bacteria. Bacterial colonies were grown over cmcagar medium1.
617 1525 1087 307 1588 1580 271 1552 1479 1159 1595 202 1472 1120 469 66 1451 1587 1393 498 327 718 546 930 411 1101 1068 802 278 729 480 570